A Recap of the 3rd MIBIREM Consortium Meeting in Prague

From June 18th to 19th, 2024, the beautiful city of Prague played host to the 3rd MIBIREM Consortium Meeting. The timing and location were meticulously chosen to coincide with the BioBio Conference 2024 and the EU Bioremediation Cluster Workshop co-organised by MIBRIEM. This strategic decision allowed us to blend our project’s discussions with broader science and industry events, enriching our exchange of ideas and fostering collaboration across the field of bioremediation and beyond.

Key Discussions and Developments

The meeting kicked off with a detailed review of the project’s progress, focusing on several crucial areas:

  • Degradation Tests: We discussed the ongoing efforts in conducting microbiome degradation tests, a pivotal component of our bioremediation goals. The conversation highlighted the challenges and successes in this area, helping to streamline our approach moving forward.
  • Enrichment Tests: Progress on microbiome enrichment tests was another key topic, with partners sharing their experiences and outcomes. This exchange of knowledge is vital as we continue to refine our methods for isolating effective microbial cultures.
  • Soil and groundwater sample access: Access to soil and groundwater samples was a point of discussion, emphasizing the importance of collaboration in ensuring that all partners have the necessary resources to conduct their research.

One of the day’s highlights was a session on environmental regulations and policies across our respective European countries. This discussion was crucial as national regulations can have a strong impact on our selection process for pilot sites where the best-performing microbial consortia will be tested.

Breakthroughs in Bioremediation Research

We were to hear about the first promising results related to microbial cultures for the biodegradation of our target contaminants: petroleum hydrocarbons (PHCs), hexachlorocyclohexane (HCH/lindane), and cyanides. Thomas Reichenauer, the scientific coordinator of MIBIREM, also presented the summary of the findings at the BioBio2024, revealing that microbial cultures have been identified with high degradation capacities for each contaminant group. These cultures are now being isolated for further characterization and use in pilot tests. Moreover, they will be deposited to facilitate their use in future bioremediation applications.

As we moved forward, the discussions naturally transitioned into the selection of pilot sites. This crucial step will allow us to demonstrate the efficacy of microbial consortia established and tested in the laboratory in real-world conditions. Breakout sessions focused on three specific use cases—cyanides, PHCs, and lindane—allowing for more targeted discussions and decision-making.

Innovation and Patenting Prospects

One of the exciting possibilities that emerged from our discussions was the potential for patenting innovative screening processes developed during the project, such as ALTAR technology. There was also talk of the potential to patent the microbiomes generated within MIBIREM, provided we can demonstrate novelty in their purpose and function, particularly in bioremediation. These discussions underscore the innovative nature of our work and its potential for broader applications beyond the scope of the project.

Moving forward

The 3rd MIBIREM Consortium Meeting was marked by insightful presentations, fruitful discussions, and significant progress on several key issues. As the meeting ended, there was a shared sense of accomplishment. We left Prague with renewed energy and a clear path forward as we continue to advance the field of bioremediation science.

 

EU Bioremediation Cluster Calls for Urgent Action in Environmental Biotechnology Research

The MIBIREM project had the honour of participating in the BioBio 2024 – the 7th International Symposium on Biosorption and Biodegradation/Bioremediation in Prague, a significant event for researchers focusing on biosorption, bioremediation, biodegradation, and broader environmental science topics such as the circular economy and green technologies.

As part of BioBio24, MIBIREM co-organized the 2nd Bioremediation Cluster Workshop, bringing together several EU projects focused on bioremediation, including MIBIREM, SYMBIOREM, BIOSYSMO, NYMPHE, EDAPHOS, and ISLANDR. The workshop was lively and engaging, featuring an opening presentation by Nicolas Kalogerakis, a renowned expert in bioremediation. We learned about the hazards of fibers and micro-nanoplastics in agriculture and explored successful methods of agricultural decontamination, such as ecopiles.

MIBIREM Project’s Promising Microbial Solutions for Contaminant Degradation

(C) RTDS: Thomas Reichenauer of AIT presenting MIBIREM at Cluster Workshop at BioBio24, 20 June 2024

Thomas Reichenauer, the scientific coordinator of MIBIREM, presented promising preliminary results on microbial cultures for the biodegradation of project target contaminants (PHCs, HCH/lindane, Cyanides) and microbiomes with high degradation capacities and excellent functionality. For all three groups of contaminants microbial cultures could be identified that show high degradation capacities. These cultures thus were selected for isolation and further characterisation of individual strains and for use in pilot-tests. The microbial cultures will be deposited to facilitate their use for future applications in bioremediation.

(c)RTDS: MIBIREM preliminary results presented at BioBio24

The workshop concluded with an engaging panel discussion, dynamically moderated by Victor De Lorenzo. The panel featured scientific coordinators from the cluster bioremediation projects, including MIBIREM, SYMBIOREMBIOSYSMONYMPHEEDAPHOS, and ISLANDR. This cluster workshop provided a promising platform for sharing knowledge and discussing the future of bioremediation in Europe. Key takeaways from the workshop include:

  • Emphasizing the importance of success stories to build public trust in research and biotechnology. Michel Chalot of Edaphos proposed installing demo sites to engage the public in technology development.
  • Highlighting the need for a holistic approach beyond bioremediation to improve overall soil health. Cosimo Masini, a MIBIREM partner of DND Biotech, stressed that soil and water are the foundation of our lives and our natural capital.
  • Addressing the gap between biotechnology and implementation, calling for greater involvement from industry and intermediary producers to ensure society benefits from scientific advancements.

A Call for Action in Environmental Biotechnology Research

A key outcome of the workshop was the creation of Joint policy documenent by the cluster participants. This document calls for urgent action in Environmental Biotechnology Research in Europe, emphasizing its relevance to human health and green technologies. It advocates for a major European-funded environmental biotechnology research initiative to enhance European research leadership, foster international collaborations, and in due course benefit all of society.

 

MIBIREM at the Circular Economy Summit 2024: Pioneering Bioremediation for a Sustainable Future

The Circular Economy Summit convened on May 28, 2024, at the eco-friendly Expedithalle in Vienna. This event brought together the circular economy community, featuring 33 high-profile speakers and attracting over 350 participants from business, politics, administration, and civil society across 10 different countries. Impeccably organized by the Circular Economy Forum Austria, the summit featured lively debates, engaging discussions, and a practical demonstration of the circular economy through an expo room showcasing various stakeholders.

Hosted by the Austrian Federal Ministry of Climate Action, Environment, Energy, Mobility, Innovation, and Technology, the summit highlighted the importance of circular economy practices in Austrian society. The event was inaugurated by the Minister for Climate Action herself, who discussed concrete measures and actions to promote a circular economy. This gathering not only showcased innovative ideas but also emphasized the crucial role of sustainability in shaping our future.

(c)RTDS: Circular Economy Summit in Vienna on 28 May 2024

MIBIREM and Circularity in Bioremediation

Circular economy principles are also central to our project. As an Austrian-led initiative, MIBIREM was among participants at the Circular Economy Summit represented by the Austrian project and communication coordinator, RTDS Group. Specializing in developing a microbiome-based toolbox for the soil remediation of contaminated sites, we are confident about the potential of our MIBIREM toolbox that aims to improve the business case for microbiome-based bioremediation.

(C): RTDS Team at the Circular Economy Summit in Vienna

MIBIREM seeks not only to start microbiome-based bioremediation but also to encourage the broader use of microbiomes in biotechnology, supporting the circular economy. By adapting our TOOLBOX for various applications, MIBIREM could enhance the use of microbiomes in biotechnology. Aligned with the European Green Deal, our project adheres to the Circular Economy Action Plan. We are facilitating a paradigm shift from single strains to microbiomes, which hold immense potential as natural, renewable resources in biotechnology.

(C): MIBIREM at the Cicular Economy Summit reperesented by RTDS 

Data is Key: Insights from the Circular Economy Summit 2024

Thanks to the vibrant circular community in Austria and the engaging Circular Economy Summit, our project gained meaningful insights into circular activities and stayed up-to-date with the latest trends in the circular economy. The event featured four insightful panels discussing EU policies, the role of data and finances, and the art of cross-border collaboration.

One of the highlights was the panel titled “Data is Key,” which included Agnieszka Sznyk from INNOWO, our bioremediation cluster partner in the Nymphe project. The panel underscored the often-overlooked importance of data in measuring the state of circularity. As Ms. Sznyk emphasized, data management is essential for circularity. To achieve or measure circularity, one must start with data—be it materials, energy, resources, waste products or something else.  There is already a vast amount of data available, and the challenge lies in using and structuring it effectively.

(C)RTDS: Circular Economy Summit panel:”Data is key”

Measuring the impact of circular activities, particularly in terms of refuse, rethink, and reduce, remains challenging. However, the discussions reaffirmed the value of all circular activities in driving the systemic change necessary for a circular economy in Austria, Europe, and globally. We left the summit reassured that these efforts are crucial for advancing a sustainable future.

 

Author: Ana Babić,

RTDS Communication and Project Manager

Upcoming Event: EU Bioremediation Projects Cluster Workshop at BioBio 2024

The Potential of Bioremediation for Clean Soils and Groundwater in Europe

Official Event of the BioBio 2024 EU Bioremediation Project Cluster

We are pleased to announce the 2nd Workshop of the EU Bioremediation Projects Cluster, taking place during BioBio 2024 – the 7th International Symposium on Biosorption and Biodegradation/Bioremediation, on 20 June 2024, in Prague, Czech Republic.

 

Launched in early 2023, our cluster includes six key bioremediation projects funded by the European Commission: MIBIREM, SYMBIOREM, BIOSYSMO, NYMPHE, EDAPHOS, and ISLANDR. This workshop will highlight the potential of bioremediation to improve soil health, clean groundwater, and enhance air quality. Bioremediation uses natural processes and microorganisms to remove contaminants without harming ecosystems.

Thomas Reichenauer, the scientific coordinator of the MIBIREM project from the AIT Austrian Institute of Technology in Austria, will share the latest advancements of the project. Other partners from the EU bioremediation cluster will also present, covering topics such as:

  • The use and release of bacteria, microbes, and GMOs for (bio-)remediation purposes – addressing environmental, toxicological, societal, and policy implications.
  • Different scientific approaches to (bio-)remediation, including classical enrichment, single strain, or artificial bacteria microbiomes.
  • Implementation and regulation barriers when considering the scale-up or large-scale implementation of various (bio-)remediation systems.

For more information read the detailed BioBio 2024 cluster workshop Agenda.

This workshop provides a platform for sharing knowledge and discussing the future of bioremediation in Europe. We look forward to connecting with everyone in Prague and working together towards a cleaner environment.

 

Soil Sampling for Degrading Microbiomes Complete

The initial phase of the MIBIREM project has recently concluded, following the completion of a sampling campaign at contaminated sites across Europe. This phase involved the search for microbiomes capable of degrading organic contaminants. The MIBIREM consortium gathered samples from soil, groundwater, and bac-traps at 13 different contaminated sites in 7 European countries.

The focus was on sites contaminated with cyanides (primarily from former gasworks), petroleum hydrocarbons (PHCs), and lindane (also known as HCH or γ-hexachlorocyclohexane) and its isomers. The samples were analyzed for physical-chemical properties and community composition based on 16S rDNA.

Soil sampling in Rinteln, Germany 2023 by (C) Sensatec

Three different contaminants

Petroleum Hydrocarbons (PHCs) are among the most common contaminants in soil and groundwater in Europe and worldwide. Finding effective microbiomes and gaining a better understanding of the environmental conditions for microbial degradation can help address environmental contamination in many locations.

Although lindane is no longer produced in Europe, localized contamination remains at production and storage sites, requiring remediation due to its high toxicity. Cyanides, previously overlooked in favor of seemingly more critical contaminants, have become a significant problem at many former gasworks across Europe.

Initial microcosm tests with samples from these sites yielded promising results, suggesting that microbiomes with high potential for degrading these contaminants are present.

 

Author: Thomas Reichenauer,

Scientific Co-ordinator, AIT

Nature-based: new opportunities in soil remediation

Development of and experience with nature-based solutions – Nature-based: new opportunities in soil remediation

A summary of the original article in the Dutch paper ‘Bodem’ (nr. 1, February 2024).

Nature-based solutions (NBS) are a crucial element in making soil and groundwater remediation more sustainable. Considering the approximately 300,000 seriously contaminated sites yet to be remediated in Europe, the use of NBS will be indispensable and will have a positive impact. Some of these techniques have already proven their value over several decades. In recent years, significant investments have been made in new techniques, variations of existing NBS and combinations of NBS with conventional techniques.

In a general sense, NBS offer several advantages over conventional high-tech techniques, such as cost-effectiveness (limited maintenance, lower energy costs, cheaper materials, less waste), reduced environmental impact, opportunities for integration with other themes (climate mitigation, biodiversity, carbon storage), and resilience in remote locations.

(c)TauwNL: Artist impression Natural CatchTAUW (Interreg RESANAT)

Certainly, there are also some focus areas and limitations of NBS, such as larger spatial requirements, longer duration, adaptation to local climates, and fluctuation of efficiency over time. Nevertheless, the exploration of possibilities and deployment of NBS for contaminated soils and surface waters are increasing due to the growing global awareness of the need for sustainability in all our activities (UN SDGs and EU Green Deal). This is evidenced by initiatives such as the EU Soil Strategy 2030 and the international standard ISO 18504 for sustainable remediation.

NBS research and development are financially supported by the EU through programs such as Horizon Europe, Interreg, and LIFE. Examples of these projects involving TAUW include GREENER (2019-2023), RESANAT (2019-2022), POPWAT (2020-2023), and MIBIREM (2022-2027). Additionally, organizations like NICOLE (Network for Industrially Coordinated Sustainable Land Management in Europe), major private stakeholders, and some government agencies (including OVAM in Flanders) are encouraging the adoption of NBS.

(c)TauwNL: Situation constructed wetland as Wetland+TM in Hájek. (Source: LIFE POPWAT, Layman’s report)

NBS aim to utilize and support natural processes, materials, and energy to facilitate the degradation, immobilization, or capture of contaminants. The mechanisms for dealing with contaminants primarily include adsorption, precipitation, biological, chemical, and photochemical degradation, as well as geohydrological isolation. Also NBS consider:

  • Utilizing the natural flow of (ground) water as a driving force for permeable reactive barriers and dispersion of admixtures.
  • Using solar energy in the form of UV radiation for photochemical degradation, heat for increasing biodegradation, and electricity for driving control units/pumps or electrochemical processes.
  • Harnessing wind for creating negative pressure for bioventing or using compressed air for biosparging.
  • Incorporating inert organic materials such as biochar, plant fibers, or peat for adsorption.
  • Employing bacteria and fungi for degradation or immobilization.
  • Utilizing trees, reeds, and other plants for phytoextraction, phytostabilization, or geohydrological control.
  • Leveraging atmospheric oxygen for chemical oxidation or aerobic biodegradation.
  • Using alkaline materials for neutralizing acidic environments, metal precipitation, and biostimulation.

EU projects such as RESANAT, GREENER, and POPWAT, along with other initiatives, have yielded initial results. Promising new NBS approaches are expected to be implemented in practice within 3-5 years, given their positive impact on the dimensions of People, Planet, and Prosperity. It’s time to take them into consideration and give them the space they deserve!

 

Author: Tobias Praamstra,

Consultant Soil, Groundwater & Sustainability, TAUW

 

Advancements in lab work thanks to new robot for soil DNA analysis

Healthy ecosystems are vital for biomass, food supply, diverse nature, water management, and human well-being. The EU and Flanders target healthy soils and water bodies, while the Air Policy Plan 2030 aims to cut air pollution’s health impact by 2030. Despite agricultural climate efforts, more research is needed, and rules are enforced for environmental impact reduction.

In this respect, our project partner Sofie Thijs, co-developed the NGSxpress DNA platform together with Interscience, Illumina, Oxford Nanopore, and Filterservice.

(c)CMK- Hasselt University lab and the NGSxpress DNA Platform, Sofie Thijs on the right

The DNA robot

What is this flexible, innovative NGSxpress platform, and what is its purpose?

● It is an automated robot and a long-read sequencer to further develop and expand various new and existing DNA analyses and applications;

● It addresses existing technical bottlenecks in DNA analyses: increasing efficiency, high throughput, accuracy, quality control, and customization;

● It allows for expanding services to Flemish (and international) companies and improving knowledge dissemination. It is a critical success factor for enabling a circular economy and mitigating the effects of climate change.

● With this purchase and installation, VLAIO/EFRO strengthens Flemish DNA expertise with applications in sectors such as healthcare, agriculture, soil biodiversity, and soil remediation.

(c) CMK- Hasselt University: DNA robot

This project is made possible thanks to the support from Europe (EFRO), VLAIO, University of Hasselt-the Centre for Environmental Sciences (CMK), Witteveen+Bos Belgium NV, and Vereecken Farms. Read more here.

Microbiome analysis, cultivation and evolution in the MIBIREM project

Sofie Thijs is a postdoc at Hasselt University, specializing in bioremediation, identification of bioactive compounds, and biotechnological applications. In the MIBIREM project, the Centre for Environmental Sciences (CMK) at Hasselt University is responsible for the microbiome analyses, cultivation, and evolution. In particular, it will use next-generation sequencing to identify bacteria with pollutant-degrading properties, trap and identify active pollutant-degrading bacteria, apply new approaches in cultivation, and improve selected microbial consortia by natural evolution. Lastly, CMK will monitor the microbiomes, bioaugmented strains, and consortia during field pilot tests.

 

Author: Sofie Thijs

Postdoc at Hasselt University

European Culture Collections’ Organisation ECCO- platform for bioremediation

Culture collections play a vital role in the conservation and sustainable use of microbial resources. They offer a range of services including the supply of quality-controlled biological material for research, teaching and valorisation in the form of reference strains. ECCO, the European Culture Collections’ organisation, organizes annual meetings since the 1980’s. These annual meetings have become a unique European platform for over 80 culture collections to convene and exchange information of scientific, managerial and practical importance.

(c) ECCO XLI in Brussels, September 2023

The objectives of the ECCO conference are to provide a platform to initiate collaborations, to exchange new scientific insights, to discuss legal developments related to culture collections, to provide examples of how culture collections can contribute to society and to discuss developments in management, and policy of microbial cultures. The themes vary widely and address aspects of micro-organisms in disease and health, food and agriculture, ecological applications, new taxonomic tools, and data management, infrastructures, career skills.

(c) Ghent University: MIBIREM at the ECCO XLI in Brussels, in September 2023

ECCO XLI was held in Brussels in September 2023. Peter Vandamme joined ECCO XLI and moderated a session on new techniques and approaches. In the frame of MIBIREM, his team aims to develop quality standards for the preservation and distribution of bioremediation consortia that are able to degrade petroleum hydrocarbons, cyanides and lindane which are all important chemical pollutants. Whilst ECCO XLI came too early to present MIBIREM results, forthcoming ECCO meetings will be a key platform to present and disseminate their findings.

 

Author: Peter Vandamme

Professor in microbiology

Faculty of Sciences, Ghent University

Soil Mission Week: Connecting People to Soil Health

The European Mission Soil Week Conference, organized by the European Commission’s Directorate-General for Agriculture and Rural Development (DG AGRI), under the Horizon Europe Mission ‘A Soil Deal for Europe’ (Mission Soil), unfolded from November 21 to 23, 2023. Hosted by the Spanish National Research Council (CSIC-INIA) in Madrid during the Spanish Presidency of the EU Council, the event saw the participation of the MIBIREM project, represented by RTDS. MIBIREM focuses on developing biotechnology for soil decontamination using bacteria and was among several soil health projects present at the conference.

The idea behind European Mission Soil Week is to raise awareness about the critical importance of soil health and translate this awareness into actionable measures for soil protection and restoration. The event served as a valuable platform to communicate innovative solutions based on the latest research findings for the promotion of healthy soils. A significant number of participants, both on-site in Madrid and online, represented various stakeholders, including the research community (most numerous), policy makers, farmers, landowners, and industry, with local communities and projects outside the scope of the Soil Health Mission initiative also in attendance.

(c)RTDS: Mission Soil Week Opening, Madrid 21 November 2023

Caring for soil is caring for life

“Caring for soil is caring for life” was a resonating theme throughout the conference. Soil health is a top priority on the EU political agenda – and for good reason. Soil is crucial in supporting human health, providing the basis for food, and sustaining vital ecosystems. Furthermore, healthy soils play a pivotal role in carbon capture, contributing to climate stability and neutrality. Astonishingly enough, approximately 60% of European soil is considered unhealthy, with 2.8 million sites potentially contaminated.

To address these challenges, various European projects, including PrepSoil, InBestSoil, Loess, ISLANDR, Solo, Edaphos, Biosysmo and MIBIREM were brought together to mobilize communities working on diverse aspects related to soil health. Additionally, numerous projects were showcased, sharing experiences, lessons learned and the knowledge gained from research in tackling soil-related issues.

The conference underscored the inseparable connection between healthy soils, food security, and climate. The significance of soil carbon to soil health was highlighted, leading to the inauguration of the international research consortium on soil carbon (IRC).

(c)RTDS: Project community coming together: MIBIREM, Biosysmo, Edaphos and InBestSoil

Speaking simple is a key starting point

Beyond scientific challenges, the conference identified common issues faced by many projects and communities engaged in soil health. Both simplifying language to make it accessible to all stakeholders and bridging the gap between scientific and policy language were emphasized.

Involving local communities more actively and communicating with a wide range of stakeholders were deemed similarly crucial. A bottom-to-top communication approach and a holistic view of the subject were identified as necessary. The importance of engaging citizens in the subject of soil health and involving a broad spectrum of stakeholders was also emphasized as vital for effectively utilizing scientific knowledge.

Knowledge and data sharing on spot

The knowledge gained from research and data sharing were highlighted as essential aspects that need to be properly facilitated. An example of this was the InBestSoil platform initiative, where over 50 participants spontaneously gathered at the venue outskirts to discuss future steps in regular meetings and engagement on data and knowledge-sharing regarding soil health. Stakeholder mapping and engagement, creating synergies in terms of information sharing and joint events were some of the topics discussed.

(c)carlottacat.com: Graphic visualisation Session Assessing Soil Health: Mission Soil Week in Madrid, 23 November 2023

Another relevant topic highlighted for MIBIREM at the conference was soil assessment and mapping. In the MIBIREM project, soil sampling is conducted within the framework of microbiome-based bioremediation. The conference emphasized the interconnected nature of soil systems, with the suggestion that a simple set of indicators should serve as a starting point. This idea was communicated by the Benchmark project, which focuses on co-creating a Soil Health Monitoring Framework. Moreover, the EU Soil Observatory (EUSO) was thoroughly presented as one of the instruments providing a comprehensive approach to European soil assessment and mapping. The conference also emphasized the need for increased data sharing and harmonization in the context of soil assessment.

What is soil to me

The eventful three days of Soil Mission Week concluded with a ceremony in the grand Spanish royal-style conference hall, featuring a photo competition award. Individuals working on soil-related subjects showcased what soil means to them by visually capturing the beauty of nature, or indeed its vulnerability to factors such as drought, degradation or contamination. This moving conclusion brought the science, funding and policies to a human and personal level, reflecting the intrinsic connection that starts off the entire journey.

 

Author: Ana Babic

RTDS Group

MIBIREM Communication Manager

Testimonial from the Bioremediation Symposium Battelle in the US

The 2023 Bioremediation Symposium, organized by Battelle and held in Austin on May 8-11th, was an outstanding event for bioremediators!

The technical details of the presentations were exceptional, as was the level of expertise of the people present. Within this framework, presenting the MIBIREM project was an honor and I have to say that we certainly were not out of place amongst the many international experts! This demonstrates that Europe is capable of achieving high levels of competence, though to keep up we will need to work hard since, on the other side of the Atlantic, a lot of funding is available for R&D activities.

(c)DND Biotech: Cosimo Masini at the 6th Int. Symposium on Bioremetiation – Battelle

During my presentation, titled ‘A Molecular Approach to Lindane Biodegradation’, the problem of this pesticide was discussed, describing some previous results obtained from a study funded by DND Biotech on samples from a contaminated site in Italy. Lindane was produced and used as a broad-spectrum insecticide and treatment against ectoparasites between 1945 and 2000. Production was inefficient because each ton of lindane resulted in the production of 8 to 12 tons of waste isomers. These waste isomers were dumped at production facilities and often led to huge landfills. More than 4.8 million tons of HCH-waste were and, for the most part, still are present worldwide. Lindane and the other HCH isomers barely degrade in the environment, bio-accumulate through the food chain, and present a risk to human health and the environment. They were banned in the EU in 2000 and placed under the Stockholm Convention on POPs in 2009.

(C)DND Biotech: Presentation of MIBIREM at Battelle on May 8, 2023

The Italian model site I described is polluted as a consequence of lindane production from the mid ‘40s to late ‘70s and two nearby disused waste disposal areas have reported high concentrations of HCH isomers (𝛼, 𝛽, 𝛾). In 2005, high concentrations of the beta isomer were detected in cow milk and dairy products from farms in the area. A state of environmental crisis was declared by the Italian government for the area and epidemiological surveillance was implemented. The two disposal areas were studied and characterized; containment plans were put in place to permanently separate the contaminated soil. However, the sediments of the river, as well as agricultural soil, have absorbed the contaminant, releasing it during flooding events. Soil and groundwater samples have been taken, characterized and used as baseline to create microbial enrichment cultures. Several fungal and bacterial strains have been isolated as able to grow on HCH as sole carbon source.

Within this framework, I introduced the Horizon Europe project MIBIREM, as a multidisciplinary venture involving experts in molecular biology, microbiology, environmental engineering, analytical chemistry, from various European research institutes, universities and SMEs. I explained the final goal of the project: the exploitation of microbiomes to implement a sustainable and cost-effective approach for widespread diffused contamination, particularly petroleum hydrocarbons, cyanides and HCH isomers. I highlighted the envisioned output of the MIBIREM project: the creation of a toolbox to identify, analyze, cultivate and upscale the microbiomes for bioremediation applications, while ensuring safety and policy alignment.

(C)DND Biotech: Cosimo Masini, MIBIREM Partner at the Battelle Symposium

(c)DND BioTech: Stand at the Bioremediation Symposium with MIBIREM flyers

Within the MIBIREM project, DND Biotech aims to be a provider of know-how in on-site, in-field bioremediation and will be responsible for the optimization and pilot implementation of the use of selected microorganisms for the removal of various contaminants (cyanides, lindane/HCH and petroleum hydrocarbons) from polluted soils. For this scope, our piloting system RoboNova will be used, providing useful data to bridge lab scale findings and field works.

Cosimo Masini

CEO of DND Biotech, MIBIREM partner